The development of the method for enzymatic hydrolysis for the extraction of toxic substances from the hair samples

Slustovskaya Yu.V.; Krys’ko M.V.; Strelova O.Yu.

doi:https://doi.org/10.17116/sudmed201760236-40

Slustovskaya Yu.V.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

Krys’ko M.V.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

Strelova O.Yu.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

The development of the method for enzymatic hydrolysis for the extraction of toxic substances from the hair samples

Authors:

Slustovskaya Yu.V., Krys’ko M.V., Strelova O.Yu.

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Journal: Forensic Medical Expertise. 2017;60(2): 36‑40

DOI: 10.17116/sudmed201760236-40

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Slustovskaya YuV, Krys’ko MV, Strelova OYu. The development of the method for enzymatic hydrolysis for the extraction of toxic substances from the hair samples. Forensic Medical Expertise. 2017;60(2):36‑40. (In Russ.)
https://doi.org/10.17116/sudmed201760236-40

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

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Abstract:

The objective of the present study was to develop and validate the method for the extraction of toxic substances from the hair samples as exemplified by enzymatic hydrolysis of barbituric acid derivatives. The experiments were carried out with the use of laboratory animals (white female rats and albino guinea pigs) that had been daily given a phenobarbital solution per os during 4 months preceding the study. The hairs obtained from the experimental animals were subjected to acid hydrolysis with a 6 mole hydrochloric acid and enzymatic hydrolysis with the use of chymopsin, trypsin, chymotrypsin, and papain solutions. The analysis of the extracted materials was performed by means of gas chromatography with mass-selective detection. The application of the proposed method for enzymatic hydrolysis produced the better results than acid hydrolysis. This technique was validated. The results of the study made possible the comparative characteristic of the effectiveness of acid and enzymatic hydrolysis.

Keywords:

barbituric acid derivatives

phenobarbital

hair

enzymatic hydrolysis

chymopsin

trypsin

chymotrypsin

papain

Authors:

Slustovskaya Yu.V.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

Krys’ko M.V.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

Strelova O.Yu.

Saint-Petersburg State Chemical and Pharmaceutical Academy, Saint-Petersburg, Russia, 197376

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