Morphological changes in the colonic muscular layer and interstitial cells of Cajal in experimental acute ulcerative colitis

Kiriukhin S.O.

FGBU "Nauchno-issledovatel'skiĭ institut morfologii cheloveka" RAMN;
Kafedra kletochnoĭ biologii i gistologii biologicheskogo fakul'teta Moskovskogo gosudarstvennogo universiteta im. M.V. Lomonosova

Makarova O.V.

Gosudarstvennaia novosibirskaia oblastnaia klinicheskaia bol'nitsa

Morphological changes in the colonic muscular layer and interstitial cells of Cajal in experimental acute ulcerative colitis

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Kiriukhin S.O., Makarova O.V.

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Journal: Russian Journal of Archive of Pathology. 2016;78(5): 27‑32

DOI: 10.17116/patol201678527-32

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To cite this article:

Kiriukhin SO, Makarova OV. Morphological changes in the colonic muscular layer and interstitial cells of Cajal in experimental acute ulcerative colitis. Russian Journal of Archive of Pathology. 2016;78(5):27‑32. (In Russ.)
https://doi.org/10.17116/patol201678527-32

Подписка 2025 (Russian Journal of Archive of Pathology)

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Objective: to investigate morphological changes in the muscular layer and an intermuscular population of interstitial cells of Cajal (ICC) in acute ulcerative colitis induced by dextran sulfate sodium (DSS). Material and methods. Acute colitis was induced by 3% DSS (molecular weight 40 kDa) in drinking water for 5 days; a control animal group was given tap water. Longitudinal colonic sections were histologically examined. Immunofluorescence staining of total preparations with antibodies to c-Kit was employed to assess an ICC population. Results. Morphological examination showed a morphological pattern of acute ulcerative colitis in the DSS-treated mice. The colonic muscular layer was thickened and the cytoplasm of smooth muscle cells was vacuolated. There was a decrease in ICC network density and counts in the muscular layer, as well as a specific c-Kit positive granularity in the ICC bodies and processes. Conclusion. In acute ulcerative colitis, there are alternative changes in the colonic muscular layer and a decrease in ICC density.

Keywords:

dextran sulfate sodium

experimental ulcerative colitis

muscular layer

interstitial cells of Cajal

Authors:

Kiriukhin S.O.

Makarova O.V.

Gosudarstvennaia novosibirskaia oblastnaia klinicheskaia bol'nitsa

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