THE EXPRESSION OF APOPTOSIS CONTROLLING PROTEINS IN PATIENTS WITH PROSTATE CANCER
M.V. Ploskonos, A.A. Nikolaev
We studied 44 tissue samples of prostate cancer from men aged 61—86 years. Heterogeneity of apoptosis protein p53, bcl-2, bax and CD95 expression in different stages of cancer and a tendency to modify expression depending on the severity of dysplastic process was revealed. The results showed a high production of the apoptosis inhibitor bcl-2, intense expression of p53, decreased bax and CD95 expression in patients with prostate cancer. The positive correlation between intensity of p53 expression and degree of tumor malignancy was revealed.
Key words: prostate cancer, apoptosis, bcl-2, bax, p53, CD95 (page 14—17).
COMPUTER MORPHOMETRY: EVALUATION OF CLINICAL SIGNIFICANCE IN ENDOMETRIAL HYPERPLASIA
A.Kh. Bishtavi, Yu.Yu. Tabakman, I.B. Manukhin
The results of computer morphometry were studied in patients with simple, complex and atypical endometrial hyperplasia. The ImaScope Color morphometric program was used. The morphometric changes in endometrial hyperplasia expressed in quantitative terms were more varied than their schematic definition in modern classification.
Key words: endometrial hyperplasia, computer morphometry (page 18—21)
HORMONAL LEVEL AND BONE MINERAL DENSITY IN WOMEN WITH DIFFERENT FORMS OF AMENORRHEA
А.G. Burduli, А.А. Gavisova, А.А. Smetnik, S.Yu. Kuznetsov, Т.Yu. Ivanets, V.P. Smetnik
The incidence of low bone mineral density in women with amenorrhea according to the form of amenorrhea, anamnesis and reproductive hormones levels is presented. The study of low bone mineral density in women with amenorrhea showed the importance of early diagnosis, preventive measures and appropriate therapy in young socially active women.
Key words: amenorrhea, FSH, androgens, bone mineral density (page 22—26).
АNTI-MÜLLERIAN HORMONE AS A DIAGNOSTIC MARKER OF POLYCYSTIC OVARY SYNDROME
E.E. Grodnitskaya, Yu.V. Scherbatyuk, I.A. Bendusov, M.A. Kurtser, G.A. Melnichenko
Serum Аnti-Müllerian hormone (AMH) in patients with polycystic ovary syndrome (PCOS) correlated with the severity of symptoms. It is an accurate marker of the antral follicle number and offers a good diagnostic potency. A cross-sectional study was performed in 62 women diagnosed with PCOS according to the ESHRE/ASRM criteria and in 67 healthy control subjects matched by age and body mass index. Serum AMH levels were significantly higher in women with PCOS. ROC curve analysis showed a cutoff point for AMH of 4.96 ng/mL (sensitivity 72.1%, specificity 90.2%) for diagnosis of PCOS. AMH has a high specificity and sensitivity as a diagnostic marker for PCOS.
Key words: аnti-Müllerian hormone, diagnostic marker, polycystic ovary syndrome (page 27—30).
LIPID METABOLISM IN PATIENTS WITH DIFFERENT PHENOTYPES OF POLYCYSTIC OVARY SYNDROME
E.V. Sheremetyeva, E.N. Andreeva, I.I. Dedov
Polycystic ovary syndrome is considered to be one of the constituent parts of a complex metabolic syndrome which predisposes to the long-term complications, such as type 2 diabetes, hypertension, dyslipidemia and coronary heart disease. Patients with PCOS need a multidisciplinary approach in the treatment and management.
Key words: polycystic ovary syndrome, metabolic syndrome, dyslipidemia, metformin (page 38—42).
SYSTEM AND LOCAL CHANGES OF L-SELECTIN AND LIGAND MEKA-79 IN PATIENTS WITH TUBAL INFERTILITY IN IVF/ICSI PROGRAMS
V.A. Burlev, N.A. Ilyasova, A.S. Onishchenko, L.N. Kuzmichev
The study showed that the implantation rate and clinical pregnancy rate in IVF/ICSI programs are higher in patients with low L-selectin level in blood serum and endometrium (superficial epithelium, glands and stroma) and with high MECA-79 expression in endometrium (luminal epithelium and glands). High L-selectin level in blood serum and endometrium (luminal epithelium and glands) and low MECA-79 expression in luminal epithelium and glands were observed in patients with IVF failure.
Key words: L-selectin, MECA-79, endometrium, IVF (page 43—50).
ASSISTING HIV-CONCORDANT COUPLES IN ART PROGRAMS
Yu.A. Solovieva, A.V. Korneev, V.V. Danilov
The number of HIV-concordant couples seeking the counseling on reproductive matters is increasing each year. This article describes the first experience of assisted reproductive technologies in HIV-concordant couples.
Key words: concordant couples, HIV, IVF, artificial insemination (page 57—58).
THE COMPARATIVE ANALYSIS OF CHROMOSOMAL ANOMALIES IN HUMAN PREIMPLANTATION EMBRYOS AND SPONTANEOUS ABORTUS
D.A. Mikitenko, L.Ya. Pilip, L.A. Spinenko, E.V. Lavrova, V.D. Zukin
The frequency and structure of chromosomal abnormalities in embryos of patients with multiple implantation failures in assisted reproduction programs were compared to those detected in the products of conception in first trimester miscarriages. Chromosomal abnormalities were detected in 43.2% of miscarriage products and in 51.01% of human blastocysts. Our results highlight the importance of preimplantation aneuploidy screening of all chromosomes in embryos of patients with multiple implantation failures in assisted reproduction cycles.
Key words: chromosomal abnormalities, preimplantation genetic diagnosis, comparative genomic hybridization (page 59—64).
THE COMPARATIVE ASSESSMENT OF EFFICACY OF IDIOPATHIC INFERTILITY THERAPY
A.A. Proskurin, E.A. Golubkin, P.A. Polivin, E.E. Kazaryan
The aim of this comparative study was to test the dietary supplement Androdoz for idiopathic pathospermia treatment compared with complex therapy with L-carnitine, zinc and vitamin A and E. The patients of group 1 (n=71) were treated with Androdoz, while the patients of group 2 (n=59) used L-carnitin, zinc, vitamin А and vitamin Е. The study showed a statistically significant increase of active sperm proportion and normal sperm concentration in Androdoz group. The study has also demonstrated good tolerance and safety of the drug.
Key words: infertility, pathospermia, Androdoz (page 65—66).
COMPARATIVE MORPHOMETRIC STUDY OF THE FETAL TESTICLES IN THE ANTENATAL PERIOD
K.V. Bunkov, A.Yu. Kuzmenkov, A.L. Savchenkov
The article presents the results of fetal testicles micromorphological study. The parenchymal-stromal ratio was analyzed and compared to identify histophysiological features and mechanism of testes development in antenatal period.
Key words: testicle, ratio, comparative analysis, morphometry (page 67—71).
DELAYED DELIVERY OF A SECOND TWIN AFTER THE PRETERM LABOR OF THE FIRST ONE IN BICHORIAL TWIN PREGNANCIES (CASE REPORT)
O.V. Makarov, P.V. Kozlov, A.S. Olenev, E.P. Ozimkovskaya, P.A. Kuznetsov, O.F. Klekovkina
Multiple pregnancy is one of the major risk factors of preterm labor. None of the current methods of preterm labor prevention is effective in multiple pregnancy. Delayed delivery of the second twin after the preterm labor of the first one can effectively reduce perinatal mortality. Three cases of successful pregnancy prolongation after the preterm labor of the first twin are reported in this article.
Key words: preterm labor, twins, prolonged pregnancy (page 72—74).
THE FOLLOW-UP AND TREATMENT OF DIFFERENTIATED THYROID CANCER AND POSTOPERATIVE HYPOPARATHYROIDISM DURING GESTATION
N.P. Makolina, N.M. Platonova, G.A. Melnichenko
This article presents the principles of follow-up and modern treatment of differentiated thyroid cancer, as well as postoperative hypoparathyroidism management during gestation and lactation.
Key words: differentiated thyroid cancer (DTC), hypothyroidism, postoperative hypoparathyroidism, pregnancy, treatment (page 75—81).
PLACENTAL EXPRESSION OF CHROMATIN PROTEINS AND THEIR POST-TRANSLATIONAL MODIFICATIONS IN COMPLICATED PREGNANCY
T.N. Pogorelova, V.O. Gunko, V.A. Linde
The contents and fractional composition of histones and non-histone proteins of chromatin placenta during physiological pregnancy and placental insufficiency were examined. The most labile fractions of histones were revealed. Decreasing of the cyclonucleotide-dependent phosphorylation activity, especially under the action of cAMP-dependent protein kinases is revealed for both types of placental nuclear proteins in complicated pregnancy. Modification of placental DNA-binding activity of a nuclear factor NF-κB is established. The role of the revealed damages of nuclear structures metabolism in placental insufficiency is discussed.
Key words: placenta, chromatin, transcription factor NF-κB, placental insufficiency (page 82—85).