E.A. Starikova
Institute of Experimental Medicine;
Pavlov First Saint Petersburg State Medical University;
Institute of Medical Education, Almazov National Medical Research Center
N.A. Podoplelova
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology
A.B. Malashicheva
Almazov National Medical Research Center;
Cytology Institute of the Russian Academy of Sciences
O.Ya. Porembskaya
Institute of Experimental Medicine;
Mechnikov North-Western State Medical University
Antithrombotic Effects of Blue Mussel Acid Hydrolysate
Journal: Journal of Venous Disorders. 2023;17(4): 302‑311
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To cite this article:
Starikova EA, Mammedova JT, Sokolov AV, Podoplelova NA, Malashicheva AB, Porembskaya OYa, Tsinzerling VA, Solovyev NV. Antithrombotic Effects of Blue Mussel Acid Hydrolysate. Journal of Venous Disorders.
2023;17(4):302‑311. (In Russ., In Engl.)
https://doi.org/10.17116/flebo202317041302
Thrombosis is a venous or arterial vascular that can sometimes cause fatal outcomes. Development of effective pharmacological agents for antithrombotic therapy is still an urgent scientific and practical objective.
To study the antithrombotic effects of blue mussel (Mytilus edulis) hydrolysate preparation.
Biochemical methods were used to analyze the effect of blue mussel hydrolysate on enzymatic activity of key serine proteases in clotting cascade. We assessed the structure of actin cytoskeleton and endothelial monolayer morphology in vitro to investigate the effect of this drug on biological activity of thrombin. Anticoagulant effect of hydrolysate was investigated in a model of posterior vena cava thrombosis in rats.
The drug inhibits serine proteinases of clotting cascade (FIIa, FXa, FXIa, FXIIa), in particular, enzymatic activity of thrombin (FIIa) against fibrinogen and synthetic substrates Tos-Gly-Pro-Arg-pNA and Ala-Ala-Arg-pNA. Anti-thrombin activity of undiluted drug was about 6.5 AT-U/ml. Hydrolysate inhibited the ability of thrombin to cause actin cytoskeleton remodeling and endothelial monolayer disintegration. Subcutaneous injections significantly changed thrombus structure and reduced the probability of clotting in a model of posterior vena cava thrombosis in rats.
M. edulis hydrolysate may be a basis for development of new drugs for therapy of hypercoagulable conditions.
Keywords:
Authors:
E.A. Starikova
Institute of Experimental Medicine;
Pavlov First Saint Petersburg State Medical University;
Institute of Medical Education, Almazov National Medical Research Center
N.A. Podoplelova
Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology
A.B. Malashicheva
Almazov National Medical Research Center;
Cytology Institute of the Russian Academy of Sciences
O.Ya. Porembskaya
Institute of Experimental Medicine;
Mechnikov North-Western State Medical University
Received:
03.10.2023
Accepted:
14.10.2023
List of references:
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