Application of the organ-on-a-chip technology in experimental ophthalmology

Close metadata

Organ-on-chip is a microfluidic device that can reproduce in vitro the minimal functional unit of an organ or system of organs and model various physiological processes and body structures with high accuracy. This review covers the main approaches to the use of the organ-on-chip technology in modern experimental ophthalmology. The analysis of literature sources revealed the following main applications of the organ-on-chip technology in ophthalmology; the technology allows modeling the anterior eye surface and its diseases, such as dry eye syndrome, as well as disorders of the posterior segment of the eye such as age-related macular degeneration, diabetic macular edema, diabetic retinopathy, glaucoma. Culturing of eye tissues in microfluidic systems helps identify the toxic effects and pharmacological activity of new compounds, and provides an opportunity for deeper understanding of the normal physiology of the eye and the pathogenesis of ocular diseases. In addition, the technology can reduce the cost and duration of experiments. Thus, the organ-on-a-chip technology has a great potential in the field of experimental ophthalmology and preclinical trials of new ophthalmic drugs.

Keywords:

organ-on-chip

tissue chip

microfluidic system

preclinical studies

retina-on-chip

cornea-on-chip

eye-on-chip

biomodelling

Authors:

S.V. Kravchenko

Krasnodar branch of S.N. Fedorov National Medical Research Center «MNTK «Eye Microsurgery»

SPIN RSCI: 7235-6295
Scopus AuthorID: 57210934930
ORCID: 0000-0003-2733-1072

V.V. Myasnikova

Kuban State Medical University;
Fedorov Intersectoral Scientific and Technical Complex “Eye Microsurgery”

SPIN RSCI: 9007-7257
Scopus AuthorID: 57208444374
ORCID: 0000-0003-1748-7962

S.N. Sakhnov

Kuban State Medical University;
Krasnodar branch of S.N. Fyodorov National Medical Research Center «MNTK «Eye Microsurgery»

SPIN RSCI: 6901-6260
Scopus AuthorID: 23499200200
ORCID: 0000-0003-2100-2972

Received:

25.07.2022

Accepted:

06.09.2022

List of references:

Tynio YaYa, Yarygina EI, Prokhorova LG, Bakutina MS, Ustinova VA. Cell culture as a model of the test system for the evaluation of the cytotoxic effect of substances of different nature. Veterinariya, zootekhniya i biotekhnologiya. 2015;(5):43-47. (In Russ.).
Kiselev AV, Sakhnov SN, Zabolotniy AG, Kalinina NYu. Cell technologies, cell therapy in ophthalmology — state and prospective. Sovremennye problemy nauki i obrazovaniya. 2018;(5):203-203. Accessed 27.07.22. (In Russ.). https://science-education.ru/ru/article/view?id=28062
Richardson L, Kim S, Menon R, Han A. Organ-on-chip technology: the future of feto-maternal interface research? Front Physiol. 2020;11:715. https://doi.org/10.3389/fphys.2020.00715
Afonicheva PK, Bulyanitsa AL, Evstrapov AA. «Organ-on-a-chip» — materials and methods of creation (review). Nauchnoe priborostroenie. 2019; 29(4):3-18. (In Russ.).
Manafi N, Shokri F, Achberger K, et al. Organoids and organ chips in ophthalmology. Ocul Surf. 2021;19:1-15. https://doi.org/10.1016/j.jtos.2020.11.004
Mylnikova AN, Kolesov DV, Moskovtsev AA, Sokolovskaya AA, Yurkiv VA, Kubatiev AA. Cellular microfluidic technologies for biomodeling of pathological processes. Pathogenesis = Patogenez. 2017;15(4):4-12. (In Russ.). https://doi.org/10.25557/GM.2018.4.9743
Alpeeva EV, Sidorenkova AF, Vorotelyak EA. Overview of cell models: from organs cultured in a petri dish to «organs-on-chips». Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2017;72(4):187-198. (In Russ.).
Campbell SB, Wu Q, Yazbeck J, Liu C, Okhovatian S, Radisic M. Beyond polydimethylsiloxane: alternative materials for fabrication of organ-on-a-chip devices and microphysiological systems. ACS biomaterials science & engineering. 2020;7(7):2880-2899. https://doi.org/10.1021/acsbiomaterials.0c00640
Zhang B, Korolj A, Lai BFL, Radisic M. Advances in organ-on-a-chip engineering. Nat Rev Mater. 2018;3(8):257-278. https://doi.org/10.1038/s41578-018-0034-7
Khalimova AA, Kovalenko AV, Paramonov GV. «Organs-on-a-chip»: evaluation of application perspectives in the pharmaceutical industry. Medical & Pharmaceutical JOURNAL «PULSE» = Mediko-farmatsevticheskiy zhurnal Pul’s. 2022;24(5):81-87. (In Russ.). https://doi.org/10.26787/nydha-2686-6838-2022-24-5-81-87
Shamojan GM, Trofimenko AI, Kade AH, et al. The influence of d-aspaphagine on the biocompatibility of the modified alginate hydrogel with the rat’s skeletal muscle tissue. Journal of Volgograd State Medical University = Vestnik Volgogradskogo gosudarstvennogo medicinskogo universiteta. 2018;1(65): 67-70. (In Russ.).
Clarke GA, Hartse BX, Niaraki Asli AE, et al. Advancement of sensor integrated organ-on-chip devices. Sensors. 2021;21(4):1367. https://doi.org/10.3390/s21041367
Zapuskalov IV, Krivosheina OI. Current trends of reconstructive surgery of traumatic damages of anterior segment of the eye (a literature review). Fyodorov Journal of Ophthalmic Surgery = Oftal’mokhirurgiya. 2013;(2):59-61. (In Russ.).
Abdalkader R, Kamei K. Multi-corneal barrier-on-a-chip to recapitulate eye blinking shear stress forces. Lab Chip. 2020;20(8):1410-1417. https://doi.org/10.1039/C9LC01256G
Peng Z, Zhou L, Wong JKW, Chan YK. Eye-on-a-chip (EOC) models and their role in the future of ophthalmic drug discovery. Exp Rev Ophthalmol. 2020;15(5):259-261. https://doi.org/10.1080/17469899.2020.1788388
Haderspeck JC, Chuchuy J, Kustermann S, Liebau S, Loskill P, et al. Organ-on-a-chip technologies that can transform ophthalmic drug discovery and disease modeling. Exp Opin Drug Discov. 2019;14(1):47-57. https://doi.org/10.1080/17460441.2019.1551873
Seo J, Byun WY, Alisafaei F, Georgescu A, et al. Multiscale reverse engineering of the human ocular surface. Nat Med. 2019;25(8):1310-1318. https://doi.org/10.1038/s41591-019-0531-2
Bai J, Wang C. Organoids and microphysiological systems: new tools for ophthalmic drug discovery. Front Pharmacol. 2020;11:407. https://doi.org/10.3389/fphar.2020.00407
Murphy P, Kabir MH, Srivastava T, et al. Light-focusing human micro-lenses generated from pluripotent stem cells model lens development and drug-induced cataract in vitro. Development. 2018;145(1):dev155838. https://doi.org/10.1242/dev.155838
Guan A, Wang Y, Phillips KS, Li Z. A contact-lens-on-a-chip companion diagnostic tool for personalized medicine. Lab Chip. 2016;16(7):1152-1156. https://doi.org/10.1039/C6LC00034G
Novikova YuP, Poplinskaya VA, Grigoryan EN. Organotypic Culturing as a Way to Study Recovery Opportunities of the Eye Retina in Vertebrates and Humans. Russian Journal of Developmental Biology. 2020;51(1):31-44. https://doi.org/10.1134/S1062360420010063
Borzenok SA, Khatsenko EI, Ostrovskiy DS, et al. The development of transplantation technique of 3D spheroids retinal pigment epithelium in the experiment on animals. Russian Journal of Transplantology and Artificial Organs = Vestnik transplantologii i iskusstvennykh organov. 2019;21(2):84-91. (In Russ.). https://doi.org/10.15825/1995-1191-2019-2-84-91
Borzenok SA, Khatsenko EI, Ostrovkiy DS, et al. The first experience of 3D spheroids retinal pigment epithelium transplantation in the experiment. Fyodorov Journal of Ophthalmic Surgery = Oftal’mokhirurgiya. 2019;(1):27-32. (In Russ.). https://doi.org/10.25276/0235-4160-2019-1-27-32
Eldred KC, Reh TA. Human retinal model systems: Strengths, weaknesses, and future directions. Devel Biol. 2021;480:114-122. https://doi.org/10.1016/j.ydbio.2021.09.001
Ragelle H, Goncalves A, Kustermann S, Antonetti DA, Jayagopal A. Organ-on-a-chip technologies for advanced blood — retinal barrier models. J Ocul Pharmacol Ther. 2020;36(1):30-41. https://doi.org/10.1089/jop.2019.0017
Jahagirdar D, Bangde P, Jain R, Dandekar P. Degenerative disease‐on‐a‐chip: Developing microfluidic models for rapid availability of newer therapies. Biotechnol J. 2021;16(10):2100154. https://doi.org/10.1002/biot.202100154
Chen LJ, Ito S, Kai H, Nagamine K, Nagai N, Nishizawa M, et al. Microfluidic co-cultures of retinal pigment epithelial cells and vascular endothelial cells to investigate choroidal angiogenesis. Sci Rep. 2017;7(1):369. https://doi.org/10.1038/s41598-017-03788-5
Chung M, Lee S, Lee B, et al. Wet‐AMD on a chip: Modeling outer blood‐retinal barrier in vitro. Adv Healthcare Mater. 2018;7(2):1700028. https://doi.org/10.1002/adhm.201700028
Maurissen TL, Pavlou G, Bichsel C, Villaseñor R, Kamm RD, Ragelle H. Microphysiological Neurovascular Barriers to Model the Inner Retinal Microvasculature. J Personal Med. 2022;12(2):148. https://doi.org/10.3390/jpm12020148
Yeste J, García-Ramírez M, Illa X, et al. A compartmentalized microfluidic chip with crisscross microgrooves and electrophysiological electrodes for modeling the blood — retinal barrier. Lab Chip. 2018;18(1):95-105. https://doi.org/10.1039/C7LC00795G
Gorshkov AN, Zaitseva MR, Snigirevskaya ES, Komissarchik YY. Functional linkage between the transport characteristics of the MDCK1 cell monolayer and their actin cytoskeleton organization. Cell Tissue Biol. 2016; 10(2):133-144. https://doi.org/10.1134/S1990519X1602005X
Wu J, Mak HK, Chan YK, et al. An in vitro pressure model towards studying the response of primary retinal ganglion cells to elevated hydrostatic pressures. Sci Rep. 2019;9(1):9057. https://doi.org/10.1038/s41598-019-45510-7

Close metadata