The significance of fibroblasts in experimental modeling of proliferative vitreoretinopathy

I.P. Khoroshilova-Maslova

FGBU "Moskovskiĭ NII glaznykh bolezneĭ im. Gel'mgol'tsa" Minzdravsotsrazvitiia Rossii

N.L. Leparskaia

FGBU "Moskovskiĭ NII glaznykh bolezneĭ im. Gel'mgol'tsa" Minzdravsotsrazvitiia Rossii

E.A. Vorotelyak

Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilova St., Moscow, Russian Federation, 119334

A.V. Vasil’ev

State budgetary healthcare facility «Chelyabinsk Regional Clinical Hospital», Chelyabinsk, Russian Federation

The significance of fibroblasts in experimental modeling of proliferative vitreoretinopathy

Authors:

I.P. Khoroshilova-Maslova, N.L. Leparskaia, E.A. Vorotelyak, A.V. Vasil’ev

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Journal: Vestnik Oftalmologii. 2017;133(5): 4‑10

DOI: 10.17116/oftalma201713354-10

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To cite this article:

Khoroshilova-Maslova IP, Leparskaia NL, Vorotelyak EA, Vasil’ev AV. The significance of fibroblasts in experimental modeling of proliferative vitreoretinopathy. Vestnik Oftalmologii. 2017;133(5):4‑10. (In Russ., In Engl.)
https://doi.org/10.17116/oftalma201713354-10

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Aim — to investigate the role of heterogeneous fibroblasts in the development of epiretinal membrane in eyes with modeled proliferative vitreoretinopathy. Material and methods. The material for investigation were 6 eyes of 3 Chinchilla rabbits. Suspended fibroblasts (fibroblasts of the human skin — 200000 cells in 0.1 ml) were injected into the vitreous cavity via the pars plana. The animals were followed up for 1 month and then made out of the experiment. The eyes were enucleated and fixed in 10% neutral buffered formalin for routine histological examination. Microscopy was performed on the Leica system. Results. The main clinical and morphological criteria for a rabbit model of PVR induced by intravitreal injection of heterogenic fibroblasts have been established: epiretinal membrane formation, changes in intraocular structures (the retinal pigment epithelium and retina), and inflammation (due to transplantation immunity). Particularities of the epiretinal membrane development and the role of different intraocular structures have been described. Conclusion. The experimental fibroblastic model of PVR reproduces the final, fibrous, stage of PVR, which is significant for efficacy evaluation of antiproliferative drugs.

Keywords:

proliferative vitreoretinopathy

fibroblasts

epiretinal membrane

retinal pigment epithelium

retina

Authors:

I.P. Khoroshilova-Maslova

FGBU "Moskovskiĭ NII glaznykh bolezneĭ im. Gel'mgol'tsa" Minzdravsotsrazvitiia Rossii

N.L. Leparskaia

FGBU "Moskovskiĭ NII glaznykh bolezneĭ im. Gel'mgol'tsa" Minzdravsotsrazvitiia Rossii

E.A. Vorotelyak

Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilova St., Moscow, Russian Federation, 119334

A.V. Vasil’ev

State budgetary healthcare facility «Chelyabinsk Regional Clinical Hospital», Chelyabinsk, Russian Federation

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